H2N2V1, Main, Exploration, bibRecord, 002125

An ELISA for detection of antibodies against influenza A nucleoprotein in humans and various animal species

Identifieur interne : 002125 ( Main/Exploration ); précédent : 002124; suivant : 002126

An ELISA for detection of antibodies against influenza A nucleoprotein in humans and various animal species

Auteurs : G. F. De Boer ; W. Back ; A. D. M. E. Osterhaus [Pays-Bas]

Source :

Archives of Virology [ 0304-8608 ] ; 1990-03-01.

RBID : ISTEX:290C12EDDC17F1C150EA17CCDBE3D71ED8F0A61A

English descriptors

Teeft :
- Allantoic fluid, Animal species, Antibody, Antibody titers, Antigen detection, Antigenic variation, Arch virol, Assay, Avian, Beringsea, Boer, Booster vaccination, Coating buffer, Days post infection, Different types, Double antibody sandwich, Elisa, Elisa buffer, Elisa titers, Experimental infection, Experimental influenza vaccines, Experimental vaccines, First vaccination, Foal, Guinea pigs, Hemagglutination inhibition, Hemagglutinin, Hemagglutinin subunit vaccine, Host specificity, Human volunteers, Immunosorbent, Immunosorbent assay, Influenza, Influenza antibodies, Influenza subtypes, Influenza virus, Influenza virus infection, Maternal antibodies, Maximum extinction, Monoclonal antibodies, Natural infections, Neuraminidase inhibition tests, Newcastle disease, Nucleoprotein, Piglet, Second vaccination, Serum samples, Split virus vaccine, Split virus vaccines, Subclinical infection, Subtypes, Subtypes sample, Subunit, Swine, Swine influenza virus, Titer, Vaccinated, Vaccination, Vaccination studies, Vaccine, Various animal species, Various strains, Vibrio cholerae filtrate, Virol, Virology, Virology department, Virus nucleoprotein, Weeks post infection, Whole virus.

Abstract

Summary: A double antibody sandwich blocking ELISA, using a monoclonal antibody (MAb) against influenza A nucleoprotein (NP) was developed to detect antibodies against influenza. Collections of serum samples were obtained from human and various animal species. All influenza A subtypes induced antibodies against hemagglutinins and NP. A close correlation between titers of the hemagglutination inhibition (HI) test and the NP-ELISA was seen. Antibodies against influenza NP were demonstrated in serum samples from humans, ferrets, swine, horses, chickens, ducks, guinea pigs, mice, and seals. The serum samples were collected at intervals during prospective epidemiological studies, from experimental and natural infections, and vaccination studies. The decline of maternal antibodies was studied in swine and horses. The NP-ELISA enables rapid serological diagnosis and is suited for influenza A antibody screening, especially in species which harbor several influenza subtypes. The HI and neuraminidase inhibition tests, however, must still be used for subtyping.

Url:

https://api.istex.fr/ark:/67375/1BB-HZHFC4MF-V/fulltext.pdf

DOI: 10.1007/BF01310622

Affiliations:

Pays-Bas

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Le document en format XML

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<term>Antigenic variation</term>
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<term>Hemagglutinin subunit vaccine</term>
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<front><div type="abstract" xml:lang="en">Summary: A double antibody sandwich blocking ELISA, using a monoclonal antibody (MAb) against influenza A nucleoprotein (NP) was developed to detect antibodies against influenza. Collections of serum samples were obtained from human and various animal species. All influenza A subtypes induced antibodies against hemagglutinins and NP. A close correlation between titers of the hemagglutination inhibition (HI) test and the NP-ELISA was seen. Antibodies against influenza NP were demonstrated in serum samples from humans, ferrets, swine, horses, chickens, ducks, guinea pigs, mice, and seals. The serum samples were collected at intervals during prospective epidemiological studies, from experimental and natural infections, and vaccination studies. The decline of maternal antibodies was studied in swine and horses. The NP-ELISA enables rapid serological diagnosis and is suited for influenza A antibody screening, especially in species which harbor several influenza subtypes. The HI and neuraminidase inhibition tests, however, must still be used for subtyping.</div>
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An ELISA for detection of antibodies against influenza A nucleoprotein in humans and various animal species

An ELISA for detection of antibodies against influenza A nucleoprotein in humans and various animal species

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri